1. Is 4BB™ MagniPhi^® DNA Polymerase supplied with dNTPs?

No. The kit only contains the enzyme and reaction buffer.

2. Is 4BB™ MagniPhi^® DNA Polymerase supplied with random primers?

No. The kit only contains the enzyme and reaction buffer.

3. What type of DNA template can be amplified by 4BB™ MagniPhi^® DNA Polymerase?

Both linear and circular DNA molecules can be amplified by 4BB™ MagniPhi^® DNA Polymerase.

4. Is 4BB™ MagniPhi^® DNA Polymerase suitable for amplification of short linear DNA fragments?

Overall, an average fragment size of 2 kb in a linear DNA sample is the lower limit. Additionally, highly degraded samples tend not to be amplified evenly across the genome.

5. Is 4BB™ MagniPhi^® DNA Polymerase suitable for the amplification of single-stranded DNA like the one obtained after reverse transcription of RNA?

Yes, 4BB™ MagniPhi^® DNA Polymerase can amplify ssDNA. The ssDNA molecules should have an average fragment size of 2 kb as the lower limit.

6. What is the smallest amount of DNA I can put into a multiple displacement amplification reaction mediated by 4BB™ MagniPhi^® DNA Polymerase?

Typically, the minimum amount of DNA for successful amplification with 4BB™ MagniPhi^® is 1 pg, although lower DNA inputs have been also successfully amplified (See “amplification results” section of the handbook). Note: amplification is dependent upon the quality of input DNA. Optimal amplification is achieved with 1 ng of DNA template. For higher amplification yields and maximum sensitivity use 4BB™ QualiPhi^® DNA polymerase (SKU: 510025 and 510100)

7. Can 4BB™ MagniPhi^® DNA Polymerase work with labelled dNTPs?

Yes, we have tested the addition of different concentrations of FITC-, Cy3- and Cy5-labelled dNTPs, achieving an efficient DNA labelling during the amplification reactions. Nevertheless, the reaction conditions will need to be optimized for each labelled dNTP.

8. Can 4BB™ MagniPhi^® DNA Polymerase be heat inactivated?

Yes, heat at 65°C for 10 minutes.