4BB™ TruePrime® Necrotic-Cell Free / Exosomal DNA Amplification Kit
The 4BB™ TruePrime® Necrotic Cell-Free / Exosomal DNA Amplification kit preferentially amplifies larger (≥1kb) DNA fragments derived through necrosis or from extracellular vesicles like exosomes. This presents a method for assessing cancer genome information regardless of tumour, with superior sensitivity (100 pg cfDNA input) and specificity.
Note: This kit cannot be used for the amplification of apoptotic cell-free DNA (160-170 bp). For this we would recommend the 4BB™ TruePrime® apoptotic cell-free DNA amplification kit.
| Features | Benefits |
|---|---|
| Specific amplification of large DNA fragments (≥1kb) | Optimized kit and protocol for large templates (time saving) |
| Excellent sensitivity and reproducibility | Perfect for precious / rare samples |
| Primer-free amplification – TthPrimPol synthesizes the primers for Phi29 DNA polymerase | No primer-associated artefacts such as primer dimers – better representivity (cost and time saving) |
| Excellent coverage with minimal bias | Reliable results for publication |
| Wide variety of cell-free DNA sample sources: • Plasma • Serum • Urine • CSF • Other bodily fluids | Less restriction in sample source – greater experimental flexibility |
| Reliable at a cfDNA input level of 100 pg, preserving SNVs and SNV frequencies across different sequencing technologies | Amplification product sequencing compatibility |
Applications:
- Assessing cancer genome information from all samples, regardless of tumour, with superior sensitivity and specificity
- Genetic research using large (≥1kb) circulating cell-free DNA
- Cancer research
- Amplification of large (≥1kb) DNA
